Quantification of Limonin Glucoside, Phytonutrient Component, in Citrus by Means of an Enzyme Immunoassay Using Anti-limonin Antiserum
نویسندگان
چکیده
منابع مشابه
Stability of limonin glucoside in beverage matrices
BACKGROUND: We completed a study over a 200-day period examining the stability of limonin glucoside formulated into three beverage matrices. RESULTS: Beverages containing limonin glucoside were found to contain limonin (0.13–20.10 mg L−1) during their initial testing; however, these concentrations were directly attributable to the presence of contaminating limonin in the particular lot of limon...
متن کاملTotal Synthesis of Limonin.
Limonoids are highly oxygenated C13α-triterpenes and common secondary metabolites. Several hundred congeners have been isolated to date. The first total synthesis of (±)-limonin, the flagship congener of the limonoids, is now reported and features 1) a tandem radical cyclization generating the BCD ring system with the C13α configuration that is essential to the limonoids and a Robinson annulati...
متن کاملDevelopment and validation of a decigram-scale method for the separation of limonin from limonin glucoside by C-18 flash chromatography
0308-8146/$ see front matter Published by Elsevier doi:10.1016/j.foodchem.2008.08.046 * Corresponding author. Tel.: +510 559 5898; fax: + E-mail address: [email protected] (A.P. A preparative method for decigram-scale polishing of limonin glucoside (1) with regard to removing traces of limonin (2), the main bitter principle in Citrus, is reported. During the method development and up-s...
متن کاملSuppression of intestinal carcinogenesis in Apc-mutant mice by limonin
Limonoids in citrus fruits are known to possess multiple biological functions, such as anti-proliferative functions in human cancer cell lines. Therefore, we aimed to investigate the suppressive effect of limonin on intestinal polyp development in Apc-mutant Min mice. Five-week-old female Min mice were fed a basal diet or a diet containing 250 or 500 ppm limonin for 8 weeks. The total number of...
متن کاملDetermination of Citrus Limonoid Glucosides by High Performance Liquid Chromatography Coupled to Post-Column Reaction with Ehrlich’s Reagent
A method for the identification and quantification of citrus limonoid glucosides in juices, based upon high performance liquid chromatography (HPLC) separation coupled to post-column reaction with Ehrlich’s reagent, has been developed. This method utilizes a phenyl stationary phase and an isocratic mobile phase. Limit of detection for the method was determined to be 50 ng of limonin glucoside. ...
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ژورنال
عنوان ژورنال: Horticultural Research (Japan)
سال: 2008
ISSN: 1347-2658,1880-3571
DOI: 10.2503/hrj.7.481